Optimization of heterologous microsatellites in Piracanjuba

Optimization of heterologous microsatellites in Piracanjuba

Blog Article

Abstract: The objective of B12 1000MCG this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus).Some markers were tested for the first time.Loci were optimized for PCR conditions and applied to a sample of 49 individuals.

Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species.From the Skin Care evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.